Mycoplasma spp. infections are often exacerbated in association with other pathogens (polymicrobial disease). Clinical signs and lesions caused by these infections are often uncharacteristic and non-specific, resulting in a complex differential diagnosis. Mycoplasmas are difficult to identify by culture, but definitive diagnosis can be facilitated by serology and molecular methods. Control of MG, MS, and MM in commercial poultry is possible under provisions of the National Poultry Improvement Plan (NPIP). In North Carolina, MG and MS are reportable diseases in commercial and non-commercial poultry.